DADES IDENTIFICATIVES | 2008_09 |
Assignatura | BIOQUÍMICA A LA NANOESCALA | Codi | 205151104 | |||||
Ensenyament |
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Cicle | 2on | |||||
Descriptors | Crèd. | Tipus | Curs | Període | ||||
5 | Obligatòria | Primer | Únic anual |
Competències | Objectius d'aprenentatge | Continguts |
Planificació | Metodologies | Atenció personalitzada |
Avaluació | Fonts d'informació | Recomanacions |
Tema | Subtema |
1- General view of Biochemistry. | Cellular, chemical and genetic fundamentals of live. Water. Glycids. Lipids. Nucleic acids. |
2- Aminoacides, peptides and proteins. | Amino acides. Peptide bond and primary structure. Three-dimensional structure of proteins. Folding and denaturalization. |
3- Methods to study proteins. | Purification and characterization. Determination of amino acidic sequence. Mass spectrometry. Determination of three-dimensional structure; X ray diffraction and nuclear magnetic resonance. |
4- Enzymes. | Enzymatic catalysis. Michaelis-Menten model. Allosteric enzymes. Enzymatic inhibition. Regulation of enzymatic activity. Catalytic strategies. General view of metabolism. |
5- Biomembranes. | Lipids as a biomembrane components. Proteins as a biomembrane components. Transport and energy transduction. Examples of biomembranes components: membrane transporters, potassium-sodium pump, ATPases, TMS transporters. |
6- Nucleic acids. | Composition and structure of DNA and RNA. Physic and chemical properties. Interactions between proteins and nucleic acids. Special nucleotides: TFOs, TFBs. Hairpins, aODNs, iRNAs, biological applications. |
7- Methods to study nucleic acids. | Electrophoresis. Nucleic acid hybridisation. Enzyme to handle nucleic acids. Polymerase chain reaction (PCR): fundamentals and applications. DNA sequencing. DNA cloning. |
8- Micro arrays. | Types. Construction: lithographic techniques. Applications: gene expression determination, genotyping, SNPs identification, specific binding to transcription factors, identification of protein interactions. |