Type A
|
Code |
Competences Specific | | A4 |
Know and understand in an integrated manner the organisms at molecular, cellular and metabolic level. |
| A10 |
Know how to apply the basic knowledge of structure and function of multicellular organisms in the production of biotechnological products. |
Type B
|
Code |
Competences Transversal | | B3 |
Critical, logical and creative thinking, and an ability to innovate |
Type C
|
Code |
Competences Nuclear |
Type A
|
Code |
Learning outcomes |
| A4 |
Use bioinformatic tools to correlate the structure (proteins and nucleic acids) of a biomolecule with its function.
Understand the mechanisms involved in genetic expression and regulation in eukaryotes and prokaryotes.
Describe the mechanisms of DNA recombination and repair and the modification-restriction systems.
Describe the organisation of information in the genome.
| | A10 |
Understand the mechanisms involved in genetic expression and regulation in eukaryotes and prokaryotes.
Describe the mechanisms of DNA recombination and repair and the modification-restriction systems.
|
Type B
|
Code |
Learning outcomes |
| B3 |
Reflect on new ways of doing things.
|
Type C
|
Code |
Learning outcomes |
Topic |
Sub-topic |
1. Introduction. |
Review of some concepts. Semiconservative mechanism of replication (Meselson-Stahl experiment). The central dogma of molecular biology. |
2. DNA replication. |
Initiation of replication in bacteria. Multiple origins of replication in eukaryotes and archaea. DNA polymerases and other enzymes involved. Replication mechanism: conductive and delayed chains (Okazaki fragments). Completion of replication. Replication of extra-chromosomal elements (viruses, plasmids and mitochondria).
|
3. DNA repair. |
Lesions in the DNA. Types of repair systems: Direct inversion, repair by excision, recombination repair, repair of base missmatchings. |
4. DNA transcription. |
Transcription to prokaryotes: initiation, elongation and termination of transcription. RNA polymerase. Promoter sequences. Completion of transcription. Transcription eukaryotes: type of RNA polymerases, transcription factors and promoters of RNA pol. I, II and III, transcription enhancers, transcription termination |
5. RNA processing and maturation. |
Post-transcriptional modifications in eukaryotic 5 'capping, splicing. Spliceosome. Alternative splicing. Trans-splicing. RNA editing. mRNA stability. Processing of rRNA and tRNA. |
6. Translation of mRNA into protein. |
Phases of translation: initiation, elongation and termination. The ribosomes. Factors of initiation, elongation and termination. tRNA and aminoacyl-tRNA synthetases. The genetic code. Chaperones and the folding of proteins. Peptide signal and transport of proteins in the endoplasmic reticulum. Post-transductional modifications. Ubiquitin and protein degradation. |
7. Regulation of the gene expression. |
Mechanisms of regulation of transcription. Regulation of the operon lac and operated trp in E. coli. Regulation by cascades of sigma factors. Regulation of gene expression in eukaryotes. Epigenetics Regulation by RNAs (riboswitchs, miRNA, siRNA).
|
Methodologies :: Tests |
|
Competences |
(*) Class hours
|
Hours outside the classroom
|
(**) Total hours |
Introductory activities |
|
1 |
0 |
1 |
Lecture |
|
44 |
42 |
86 |
Problem solving, classroom exercises |
|
15 |
5 |
20 |
Personal tuition |
|
1 |
0 |
1 |
|
Mixed tests |
|
3 |
39 |
42 |
|
(*) On e-learning, hours of virtual attendance of the teacher. (**) The information in the planning table is for guidance only and does not take into account the heterogeneity of the students. |
Methodologies
|
Description |
Introductory activities |
Activities designed to make contact with students, collect information from them and introduce the subject. |
Lecture |
Description of the contents of the subject. |
Problem solving, classroom exercises |
Formulation, analysis, resolution and debate of a problem or exercise related to the topic of the subject. |
Personal tuition |
Resolution of doubts and queries. |
Description |
Face-to-face in the office 107 of the Department of Biochemistry and Biotechnology, prior consultation by e-mail: santi.garcia-vallve@urv.cat |
Methodologies |
Competences
|
Description |
Weight |
|
|
|
|
Problem solving, classroom exercises |
|
Problem solving, exercises in the classroom by the students with the assistance of the teachers. |
15% |
Mixed tests |
|
Two partial exams. |
85% |
Others |
|
|
|
|
Other comments and second exam session |
The second call consists of a single exam. The grade of the problems will be maintained for the second call evaluation. During evaluation tests, mobile phones, tablets and other devices that are not expressly authorized by the test must be switched off and out of sight. The demonstration of fraudulent conduct of some evaluative activity of a subject in both material: virtual and electronic support, leads to the student the fail of this evaluation activity. Regardless of this, in view of the seriousness of the facts, the centre may propose the initiation of a disciplinary file, which will be initiated by resolution of the rector. |
Basic |
[edited by] Jocelyn E. Krebs, Elliott S. Goldstein, Stephen T. Kilpatrick, Lewin's Genes XI, 11a o la darrera disponible, Jones & Bartlett Learning, cop. 2014
J.D. Watson, T.A. Baker, A. Gann, M. Levine, R. Losick, Molecular Biology of the gene, 7a o la darrera disponible, Pearson, cop. 2013
B.E. Tropp., Molecular Biology: Genes to Proteins., 4a o la darrera disponible , Jones & Bartlett Learning, cop. 2012
|
|
Complementary |
Jocelyn E. Krebs, Elliott S. Goldstein, Stephen T. Kilpatrick, Lewin's essential genes., , Jones and Bartlett Publishers, cop. 2013
|
|
Subjects that are recommended to be taken simultaneously |
STRUCTURE AND FUNCTION OF BIOMOLECULES/19204107 |
|
Subjects that it is recommended to have taken before |
BIOCHEMISTRY/19204008 | CELL BIOLOGY/19204006 | BIOLOGY/19204005 |
|
(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation. |
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