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Type A
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Code |
Competences Specific | | | CE10 |
Appreciate the complexity and diversity of organisms through the study of their molecules, cells, physiological processes, genetics and evolution. |
| | CE11 |
Appreciate the speed of potential changes in Biochemistry. |
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Type B
|
Code |
Competences Transversal |
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Type C
|
Code |
Competences Nuclear |
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Type A
|
Code |
Learning outcomes |
| | CE10 |
Understand how oncogenes are able to initiate tumor growth.
Understand the biological significance of DNA repair and recombination processes and their importance in the evolution of genomes.
Know how information is organized in the eukaryotic nuclear genome and become aware of the limitations of our knowledge and the speed of change.
Know the basic tools of recombinant DNA technology, molecular cloning and the construction of genotheques.
Be aware that discoveries in molecular genetics have made possible the development of recombinant DNA technology.
Be able to discuss the potential effects of mutations depending on the specific site of the DNA sequence in which they occur.
Be able to discuss the importance of epigenetic mechanisms that can modulate the message expression of the DNA sequence. Know that DNA methylation can be influenced by environmental factors such as diet.
| | | CE11 |
Know how information is organized in the eukaryotic nuclear genome and become aware of the limitations of our knowledge and the speed of change.
Know the basic tools of recombinant DNA technology, molecular cloning and the construction of genotheques.
Be aware that discoveries in molecular genetics have made possible the development of recombinant DNA technology.
Be able to discuss the importance of epigenetic mechanisms that can modulate the message expression of the DNA sequence. Know that DNA methylation can be influenced by environmental factors such as diet.
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Type B
|
Code |
Learning outcomes |
|
Type C
|
Code |
Learning outcomes |
| Topic |
Sub-topic |
| 1. Introduction. |
Organization of the subject. Basic concepts of Molecular Genetics and Genetic Engineering. |
| 2. Manipulation and characterization of DNA and RNA in vitro. |
Chemical synthesis of DNA and RNA. DNA and RNA polymerases. PCR. DNA and RNA sequencing. Restriction enzymes. Ligases. DNA marking. Southern, Northern. DNA microarrays. |
| 3. Molecular Cloning in Escherichia coli. |
Vectors for the amplification and expression of genes in E. coli: plasmids and bacteriophages. Use of DNA in E. coli recombinant selection. Modification and expression of cloned genes. Genomic library and expression libraries. Expression of recombinant proteins in E. coli. |
| 4. Genetic engineering in Saccharomyces cerevisiae yeast. |
|
| 5. Classical genetic engineering in plants. |
|
| 6. Classical genetic engineering in animal cells and organisms. |
|
| 7. Editing eukaryotic genomes using CRISPR / Cas9 technology. |
|
| 8. Manipulating gene expression in eukaryotes using small silencing RNAs. |
|
| 10. Organizing the genome in model eukaryotic organisms. |
|
| 12. Genetic variations and genetic diseases. |
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| Methodologies :: Tests |
| |
Competences |
(*) Class hours
|
Hours outside the classroom
|
(**) Total hours |
| Introductory activities |
|
4 |
0 |
4 |
| Lecture |
|
24 |
36 |
60 |
| Laboratory practicals |
|
15 |
22.5 |
37.5 |
| Problem solving, exercises |
|
15 |
22.5 |
37.5 |
| Personal attention |
|
0 |
4 |
4 |
| |
| Mixed tests |
|
2 |
4 |
6 |
| |
(*) On e-learning, hours of virtual attendance of the teacher.
(**) The information in the planning table is for guidance only and does not take into account the heterogeneity of the students. |
Methodologies
| |
Description |
| Introductory activities |
Activities aimed at contacting and collecting information from students and presentation of the subject. |
| Lecture |
Sessions where the teacher explains the main content of the subject |
| Laboratory practicals |
Apply, at a practical level, the theory in a given context. Practical exercises through the different laboratories. |
| Problem solving, exercises |
Work in class and at home on problems related to the subject. Moodle questionnaires. |
| Personal attention |
Time that each teacher has reserved to attend and resolve doubts to students. |
|
Description |
|
Time each teacher has reserved to attend and answer questions to students. Due to the health emergency, student care may be carried out through online meetings, at previously arranged times by email, or through other virtual tools.
|
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Methodologies |
Competences
|
Description |
Weight |
|
|
|
|
| Laboratory practicals |
|
Prior preparation, active participation, attendance and motivation, preparation of a report of results and achievement of knowledge will be evaluated through short questions or problem solving. |
25% |
| Problem solving, exercises |
|
Problem solving in the classroom, participation in problem sessions, solving questionnaires in Moodle |
25% |
| Mixed tests |
|
It will consist of an exam of the mixed tests type, which will include all the content worked on in the subject |
50% |
| Others |
|
|
|
| |
|
Other comments and second exam session |
|
Internships are compulsory. In order to weigh all the sections, a minimum grade of 50% of each part will be required. The second call will consist of a mixed test where all the contents of the subject will be evaluated. A minimum grade of 50% is required. The grade obtained during the course of problems and practices will be saved. During the assessment tests, mobile phones, tablets and other devices not expressly authorized for the test must be switched off and out of sight. The demonstratively fraudulent realization of some evaluative activity of some asignatura so much in material support as virtual and electronic carries to the student the note of suspense of this evaluative activity. Regardless of this, given the gravity of the facts, the center may propose the initiation of a disciplinary proceeding, which will be initiated by resolution of the rector. |
| Basic |
Primose S.B. and Twyman R.M, PRINCIPLES OF GENE MANIPULATION AND GENOMICS, Útima edición disponible en CRAI Sescelades,
Terry Brown, Gene cloning and DNA analysis, Útima edición disponible.,
Greg Gibson; Spencer V. Muse , A Primer of Genome Science , Útima edición disponible.,
Krebs, JE, Goldstein ES, Kilpatrick ST, Lewin's Essential GENES, Útima edición disponible.,
|
|
| Complementary |
Peter Sudbery , Genética Molecular humana , Útima edición disponible en CRAI Sescelades. ,
Philip Meneely , Advanced Genetic Analysis , Útima edición disponible.,
Lewin B, GENES X, Útima edición disponible.,
Watson J.D. et al, BIOLOGÍA MOLECULAR DEL GEN, Útima edición disponible.,
|
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| Subjects that continue the syllabus |
| METHODOLOGY AND EXPERIMENTATION IN MOLECULAR BIOSCIENCES I/13214121 | | EPIGENETICS/13214216 |
|
| Subjects that are recommended to be taken simultaneously |
| METHODOLOGY AND EXPERIMENTATION IN MOLECULAR BIOSCIENCES I/13214121 |
|
| Subjects that it is recommended to have taken before |
| GENETICS/13214007 | | GENE EXPRESSION AND REPLICATION/13214102 |
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(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation. |
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