IDENTIFYING DATA 2021_22
Subject (*) CELL CULTURES AS AN EXPERIMENTAL MODEL FOR NUTRITIONAL STUDIES Code 13675212
Study programme
Nutrition and Metabolism (2012)
 Cycle 2nd
Descriptors Credits Type Year Period
3 Optional AN
Language
Castellà
Department Biochemistry and Biotechnology
Coordinator
AROLA ARNAL, ANNA
 E-mail anna.arola@urv.cat
juanjose.moreno@urv.cat
ruth.ferrer@urv.cat
montserrat.mitjans@urv.cat
raquel.martin@urv.cat
Lecturers
AROLA ARNAL, ANNA
MORENO AZNÁREZ, JUAN JOSÉ
FERRER ROIG, RUTH
MITJANS ARNAL, MONTSERRAT
MARTÍN VENEGAS, RAQUEL
Web http://www.ub.edu/grad3/plae/AccesInformePD?curs=2021&codiGiga=569335&idioma=CAT&recurs=publicacio
General description and relevant information Subject taught by the UB. This course aims to introduce the students to the morphological and physiological characteristics of the intestinal epithelium, the fundaments of cell behaviour in cultures and basic techniques associated with cell cultures, the rules that regulate the work in a laboratory of cell cultures and basic strategies for designing experimental cell cultures.

Competences
Type A Code Competences Specific
  Common
  Professional
  Research
Type B Code Competences Transversal
  Common
Type C Code Competences Nuclear

Learning aims
Objectives Competences

Contents
Topic Sub-topic
1. Intestinal barrier function. Experimental design for the study of barrier epithelial function.
 Barrier function components. Paracellular and transcellular pathway Narrow scaffolds. Experimental design for the study of epithelial barrier function. Bookmarks of the paracellular route. Determination of the transepithelial electrical resistance.
2. Pathways and mechanisms for intestinal absorption of nutrients. Basis of experimental models.
 Simple diffusion. Equilibrative and concentrative mediated transport. Methodologies in vivo and in vitro for the study of intestinal absorption. Advantages and limitations. Experimental design for the study of trans-cellular transport. Role of dietary components on the absorption of nutrients and epithelial barrier function.
3. Biology of cell cultures. Type of cell cultures. Basis of cell cultures used in nutritional studies: an approach to the physiology of the digestive system. Intestinal cell lines: Caco-2, HT-29, IEC-6 y T84.
4. Infrastructure, equipment and cell culture media.
 Cell cultures facilities. Requirements for cell culture laboratories. Equipment for handling and observation of cells in cultures. Cell culture media: minimum requirements and supplementation. Support for cultures.
5. Basic techniques of cell cultures. How to work in the crop room. Plating, propagation, maintenance, division and conservation of cell lines. Characterization and differentiation strategies.

Planning
Methodologies  ::  Tests
  Competences (*) Class hours Hours outside the classroom (**) Total hours
Introductory activities
0 0 0
 
 
Personal attention
0 0 0
 
Multiple-choice objective tests
0 0 0
 
(*) On e-learning, hours of virtual attendance of the teacher.
(**) The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies
  Description
Introductory activities Activities to make contact and to collect information from students. Course presentation.
Personal attention

Personalized attention
 
Personal attention
Description
Raquel Martín Venegas raquelmartin@ub.edu

Assessment
  Description Weight
 
Other comments and second exam session


Sources of information

Basic

Complementary

Recommendations
(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation.