IDENTIFYING DATA 2017_18
Subject (*) GENE EXPRESSION AND REPLICATION Code 19204102
Study programme
Bachelor's Degree in Biotechnology (2009)
 Cycle 1st
Descriptors Credits Type Year Period
6 Compulsory Second 2Q
Language
Català
Department Biochemistry and Biotechnology
Coordinator
SALVADÓ ROVIRA, MARIA JOSEPA
 E-mail mariajosepa.salvado@urv.cat
Lecturers
SALVADÓ ROVIRA, MARIA JOSEPA
Web
General description and relevant information Study of the mechanisms and regulation of DNA replication, transcription of DNA to RNA, and translation of mRNA to proteins to viruses, prokaryotes and eukaryotes.

Competences
Type A Code Competences Specific
 A4 Know and understand in an integrated manner the organisms at molecular, cellular and metabolic level.
 A10 Know how to apply the basic knowledge of structure and function of multicellular organisms in the production of biotechnological products.
Type B Code Competences Transversal
 B3 Critical, logical and creative thinking, and an ability to innovate
Type C Code Competences Nuclear

Learning outcomes
Type A Code Learning outcomes
 A4 Use bioinformatic tools to correlate the structure (proteins and nucleic acids) of a biomolecule with its function.
Understand the mechanisms involved in genetic expression and regulation in eukaryotes and prokaryotes.
Describe the mechanisms of DNA recombination and repair and the modification-restriction systems.
Describe the organisation of information in the genome.
 A10 Understand the mechanisms involved in genetic expression and regulation in eukaryotes and prokaryotes.
Describe the mechanisms of DNA recombination and repair and the modification-restriction systems.
Type B Code Learning outcomes
 B3 Reflect on new ways of doing things.
Type C Code Learning outcomes

Contents
Topic Sub-topic
1. Introduction. Review of some concepts. Semiconservative mechanism of replication (Meselson-Stahl experiment). The central dogma of molecular biology.
2. DNA replication. Initiation of replication in bacteria. Multiple origins of replication in eukaryotes and archaea. DNA polymerases and other enzymes involved. Replication mechanism: conductive and delayed chains (Okazaki fragments). Completion of replication. Replication of extra-chromosomal elements (viruses, plasmids and mitochondria).
3. DNA repair. Lesions in the DNA. Types of repair systems: Direct inversion, repair by excision, recombination repair, repair of base missmatchings.
4. DNA transcription. Transcription to prokaryotes: initiation, elongation and termination of transcription. RNA polymerase. Promoter sequences. Completion of transcription. Transcription eukaryotes: type of RNA polymerases, transcription factors and promoters of RNA pol. I, II and III, transcription enhancers, transcription termination
5. RNA processing and maturation. Post-transcriptional modifications in eukaryotic 5 'capping, splicing. Spliceosome. Alternative splicing. Trans-splicing. RNA editing. mRNA stability. Processing of rRNA and tRNA.
6. Translation of mRNA into protein. Phases of translation: initiation, elongation and termination. The ribosomes. Factors of initiation, elongation and termination. tRNA and aminoacyl-tRNA synthetases. The genetic code. Chaperones and the folding of proteins. Peptide signal and transport of proteins in the endoplasmic reticulum. Post-transductional modifications. Ubiquitin and protein degradation.
7. Regulation of the gene expression. Mechanisms of regulation of transcription. Regulation of the operon lac and operated trp in E. coli. Regulation by cascades of sigma factors. Regulation of gene expression in eukaryotes. Epigenetics Regulation by RNAs (riboswitchs, miRNA, siRNA).

Planning
Methodologies  ::  Tests
  Competences (*) Class hours
 Hours outside the classroom
 (**) Total hours
Introductory activities
A2
A4
A9
 1 0 1
Lecture
A2
A4
A9
 44 42 86
Problem solving, classroom exercises
A2
A4
A9
B3
 15 5 20
Personal tuition
1 0 1
 
Mixed tests
A4
A10
 3 39 42
 
(*) On e-learning, hours of virtual attendance of the teacher.
(**) The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies
  Description
Introductory activities Activities designed to make contact with students, collect information from them and introduce the subject.
Lecture Description of the contents of the subject.
Problem solving, classroom exercises Formulation, analysis, resolution and debate of a problem or exercise related to the topic of the subject.
Personal tuition Resolution of doubts and queries.

Personalized attention
Description
Face-to-face in the office 107 of the Department of Biochemistry and Biotechnology, prior consultation by e-mail: santi.garcia-vallve@urv.cat

Assessment
Methodologies Competences Description Weight        
Problem solving, classroom exercises
A2
A4
A9
B3
 Problem solving, exercises in the classroom by the students with the assistance of the teachers. 15%
Mixed tests
A4
A10
 Two partial exams. 85%
Others  
 
Other comments and second exam session

The second call consists of a single exam. The grade of the problems will be maintained for the second call evaluation.

During evaluation tests, mobile phones, tablets and other devices that are not expressly authorized by the test must be switched off and out of sight.

The demonstration of fraudulent conduct of some evaluative activity of a subject in both material: virtual and electronic support, leads to the student the fail of this evaluation activity. Regardless of this, in view of the seriousness of the facts, the centre may propose the initiation of a disciplinary file, which will be initiated by resolution of the rector.

Sources of information

Basic [edited by] Jocelyn E. Krebs, Elliott S. Goldstein, Stephen T. Kilpatrick, Lewin's Genes XI, 11a o la darrera disponible, Jones & Bartlett Learning, cop. 2014
J.D. Watson, T.A. Baker, A. Gann, M. Levine, R. Losick, Molecular Biology of the gene, 7a o la darrera disponible, Pearson, cop. 2013
B.E. Tropp., Molecular Biology: Genes to Proteins., 4a o la darrera disponible , Jones & Bartlett Learning, cop. 2012

Complementary Jocelyn E. Krebs, Elliott S. Goldstein, Stephen T. Kilpatrick, Lewin's essential genes., , Jones and Bartlett Publishers, cop. 2013

Recommendations

Subjects that are recommended to be taken simultaneously
STRUCTURE AND FUNCTION OF BIOMOLECULES/19204107

Subjects that it is recommended to have taken before
BIOCHEMISTRY/19204008
CELL BIOLOGY/19204006
BIOLOGY/19204005
(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation.