IDENTIFYING DATA 2020_21
Subject (*) TECHNIQUES IN BIOCHEMISTRY AND MOLECULAR BIOLOGY Code 19204104
Study programme
Bachelor's Degree in Biotechnology (2009)
 Cycle 1st
Descriptors Credits Type Year Period
6 Compulsory Third 1Q
Language
Català
Department Biochemistry and Biotechnology
Coordinator
BLAY OLIVÉ, MARIA TERESA
 E-mail mteresa.blay@urv.cat
elsa.maymo@urv.cat
neus.martinez@urv.cat
mariadelmar.rodriguez@urv.cat
Lecturers
BLAY OLIVÉ, MARIA TERESA
MAYMO MASIP, ELSA
MARTÍNEZ MICAELO, NIEVES BEATRIZ
RODRIGUEZ PEÑA, MARIA DEL MAR
Web http://moodle.urv.net
General description and relevant information Should there be a health emergency that requires the general public to be confined or which involves restricted mobility during the academic year, we shall attempt to adapt teaching and assessment. Should this situation arise, information about any changes will be given on the Moodle space for every subject. Seminars on different biochemical and molecular biology techniques.

Competences
Type A Code Competences Specific
 A5 Know the principles, the instrumentation and the applications of the main techniques of analysis and separation of biomolecules, as well as the techniques of culture of microorganisms and cells of multicellular organisms.
 A6 Know how to design and apply experimental laboratory protocols in the fields of biotechnology, especially chemical, biochemical, microbiological and molecular biology, assessing their risks and safety elements.
 A8 Analyse appropriately data and experimental results from the fields of biotechnology with statistical techniques and be able to interpret it.
Type B Code Competences Transversal
Type C Code Competences Nuclear

Learning outcomes
Type A Code Learning outcomes
 A5 Know and master the instrumental analytical techniques that apply to biotechnology.
 A6 Acquire experience in the oral presentation and defense of a task.
 A8 Know how to interpret data and experimental results.
Present and discuss the results obtained.
Type B Code Learning outcomes
Type C Code Learning outcomes

Contents
Topic Sub-topic
1. Work levels when focusing on a biochemical/biotechnological study.
2. Initial processing of biological samples.
3. Separation techniques of cells and cellular components.
4. Techniques related to the use of radioisotopes.
5. Nucleic acid and protein quantification techniques.
6. Specific techniques of molecular biology.
7. Immunological techniques frequently used in biochemistry and biotechnology.
8. Other techniques.

Planning
Methodologies  ::  Tests
  Competences (*) Class hours
 Hours outside the classroom
 (**) Total hours
Introductory activities
1 0 1
Lecture
CE5
CE6
 14 21 35
Seminars
CE5
CE6
 14 21 35
Laboratory practicals
CE6
CE8
 14 21 35
Personal attention
1 0 1
 
Mixed tests
A5
A6
A8
 2 0 2
 
(*) On e-learning, hours of virtual attendance of the teacher.
(**) The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies
  Description
Introductory activities Activities aimed to making contact and collecting information from students and presentation of the subject.
Lecture Presentation by the teacher of the different contents of the subject.
Seminars In-depth work by students on different sections of the contents of the subject.
Laboratory practicals Practical application of a selection of the techniques worked in the seminars and lectures sessions.
Personal attention Reserved time to contact directly with the students outside the class, to solve queries and guide them in the learning process.

Personalized attention
Description
Individualized teacher-student interaction outside the classroom, in order to solve queries and guide in the learning of the subject.

Assessment
Methodologies Competences Description Weight        
Seminars
CE5
CE6
 Evaluation of an oral presentation. 40
Laboratory practicals
CE6
CE8
 Evaluation of an internship report 20
Mixed tests
A5
A6
A8
 Assessment of the master's session contents. 40
Others  
 
Other comments and second exam session

The second call consists of a mixed test which collect all the theoretical contents of the subject with a weight of 40%.

The grades of the seminars and practices are saved for the 2nd call.

The demonstration of the fraudulent conduct of some evaluative activity of a subject in both material and virtual or electronic support leads to the student the failing grade of this evaluation activity. Regardless of this, in view of the seriousness of the facts, the centre may propose the initiation of a disciplinary file, which will be initiated by resolution of the rector.

Sources of information

Basic Ausubel, Current Protocols in molecular biology, , John Willey & Sons
Alberts, Biología molecular de la célula, 3ª, Edicions Omega
Williams & Wilson, Principios y técnicas de Bioquímica experimental, 5th, Cambridge University Press
Holme & Peck, Analytical Biochemistry, , Longman Group
Davis, Basic cell culture:a practical approach, , IRL Press
Harlow, Antibodies, ,

Complementary

Recommendations


Subjects that it is recommended to have taken before
GENETICS/19204007
GENE EXPRESSION AND REPLICATION/19204102
(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation.