IDENTIFYING DATA 2019_20
Subject (*) ENZYMOLOGY Code 19204106
Study programme
Bachelor's Degree in Biotechnology (2009)
 Cycle 1st
Descriptors Credits Type Year Period
6 Compulsory Second 1Q
Language
Català
Department Biochemistry and Biotechnology
Coordinator
GARCIA VALLVE, SANTIAGO
 E-mail santi.garcia-vallve@urv.cat
veronica.arreaza@urv.cat
miriam.ejarque@urv.cat
irene.cano@urv.cat
Lecturers
GARCIA VALLVE, SANTIAGO
ARREAZA GIL, VERONICA
EJARQUE CARBO, MIRIAM
CANO TORRES, IRENE
Web http://moodle.urv.net/moodle/
General description and relevant information In this subject, the student acquires the essential knowledge on enzymes as catalysts in the biological processes. Given that the enzymatic action intervenes in the kinetics of the reactions, an important part of the subject is centred in the study of enzymatic kinetics and the conditioners of the reaction rate. It is also intended to develop the basic techniques of protein purification.

Competences
Type A Code Competences Specific
 A3 Have a good knowledge of biochemistry, enzyme kinetics and mechanisms, metabolism and its regulation.
 A6 Know how to design and apply experimental laboratory protocols in the fields of biotechnology, especially chemical, biochemical, microbiological and molecular biology, assessing their risks and safety elements.
Type B Code Competences Transversal
Type C Code Competences Nuclear

Learning outcomes
Type A Code Learning outcomes
 A3 Know the general properties of the enzymes, their nomenclature and classification, the strategies of their purification, and the basic principles of kinetics and enzymatic catalysis.
 A6 Use bioinformatic tools to correlate the structure (proteins and nucleic acids) of a biomolecule with its function.
Type B Code Learning outcomes
Type C Code Learning outcomes

Contents
Topic Sub-topic
1. Introduction Enzyme definition. Historical perspective. Main features of enzymes. Cofactors. Nomenclature and classification of enzymes.
2. Enzymatic kinetics Definition of reaction rate. Measurement of enzymatic activity. Enzymatic kinetics of the reactions of a substrate. Enzymatic inhibition in the reactions of a substrate. Effects of pH and temperature. Enzymatic kinetics of the reactions of two or more substrates.
3. Enzymatic purification Objectives. Purification strategies. Methods of separation. Choice of purification methodology. Follow-up purification. Measurement of the success of a purification. Examples of purification.
4. Mechanisms of enzymatic action Catalysis bases. Effect of proximity and orientation. Acid-base catalysis. Catalysis by metal ions. Covalent catalysis. Examples of enzymatic mechanisms. Experimental determination of enzymatic mechanisms.
6. Regulation of enzyme activity Mechanisms of regulation of enzymatic activity. Homotrophic and heterotropic cooperative effects. Allosteric enzymes. Models that explain the cooperative and allosteric character.

Planning
Methodologies  ::  Tests
  Competences (*) Class hours
 Hours outside the classroom
 (**) Total hours
Introductory activities
1 0 1
Lecture
CE3
CE6
 28 42 70
Problem solving, exercises
1 9 10
Laboratory practicals
A3
 12 19 31
Problem solving, exercises in the classroom
A3
A6
 13 20 33
Personal attention
1 0 1
 
Mixed tests
A3
A6
 4 0 4
 
(*) On e-learning, hours of virtual attendance of the teacher.
(**) The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies
  Description
Introductory activities Activities designed to make contact with students, collect information from them and introduce the subject.
Lecture Description of the contents of the subject.
Problem solving, exercises Formulation, analysis, resolution and debate of a problem or exercise related to the topic of the subject.
Laboratory practicals Practical application of the theory of a knowledge area in a particular context. Practical exercises in the different laboratories.
Problem solving, exercises in the classroom Formulation, analysis, resolution and debate of a problem or exercise related to the topic of the subject.
Personal attention Attention to any question raised by students.

Personalized attention
Description
Attention to any question raised by students in the office 105 of the Department of Biochemistry and Biotechnology or sending an e-mail to anna.arola@urv.cat.

Assessment
Methodologies Competences Description Weight        
Problem solving, exercises
Resolution and evaluation of problems and exercises by the student.
 10%
Laboratory practicals
A3
 To pass the subject the attendance is compulsory.
Examination at the end of the laboratory practicals period and delivery of the proposed activities.
It is required a minimum grade of 5/10 of the laboratory practicals exam to pass the subject.
 15%
Mixed tests
A3
A6
 Two partial exams of objective tests and that will also include problems and exercises to solve. It will evaluate all the content worked in the subject. It is required an average grade of the two partial exams with a minimum grade of 5/10 to pass the subject and to count the grade of the other evaluation activities. It is an essential requirement to average the partial and pass the subject a minimum grade of 4/10 in each partial exam and there is only one opportunity to perform each of the tests. 75%
Others  
 
Other comments and second exam session

Second call: it consists of a single examination of the whole contents of the subject of objective tests and it includes problems and exercises to solve too. The exam has a weight of 75% of the final mark and a minimum grade of 5/10 it is necessary in the exam to pass the subject and to count the grade of the rest of evaluation activities (laboratory practices and problem solving and exercises). The rest of the activities keep the notes and the grade obtained in the first call, except for those who have a lower mark than 5/10 in the laboratory practice exam, which have to repeat in the second call.


During evaluation tests, mobile phones, tablets and other devices that are not expressly authorized for the test must be switched off and out of sight.
The demonstration of fraudulent conduct of some evaluative activity of a subject in both material: virtual and electronic support, leads to the student the fail of this evaluation activity. Regardless of this, in view of the seriousness of the facts, the centre may propose the initiation of a disciplinary file, which will be initiated by resolution of the rector.

Sources of information

Basic Núñez de Castro, I. , Enzimología., Última edició disponible, Pirámide
Palmer, T., Understanding enzymes, Última edició disponible, Prentice Hall/Ellis Horwo
Price, N. C.; Stevens, L., Fundamentals of enzymology : the cell and molecular biology of catalytic proteins., Última edició disponible, Oxford University Press.
Nelson, D. K.; Cox, M. M., Lehninger principios de bioquímica, Última edició disponible, Barcelona: Omega, cop.
Donald Voet, Judith G. Voet, Charlotte W. Pratt, , Fundamentos de bioquímica : la vida a nivel molecular, Última edició disponible, Médica Panamericana

Complementary de Arriaga M.D.; Soler J.; et al , Manual de ejercicios de cinética enzimática, Última edició disponible, Universidad de León. Secretariado de Public
Mathews, C. K.; Van Holde; K. E., Ahern, K. G., Bioquímica, Última edició disponible, Addison Wesley
, http://www.expasy.org/enzyme/, ,

Recommendations

Subjects that continue the syllabus
INDUSTRIAL ENZYMOLOGY/19204217

Subjects that are recommended to be taken simultaneously
STRUCTURE AND FUNCTION OF BIOMOLECULES/19204107

Subjects that it is recommended to have taken before
BIOCHEMISTRY/19204008
(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation.