IDENTIFYING DATA 2020_21
Subject (*) ENZYMOLOGY Code 19204106
Study programme
Bachelor's Degree in Biotechnology (2009)
 Cycle 1st
Descriptors Credits Type Year Period
6 Compulsory Second 1Q
Language
Català
Department Biochemistry and Biotechnology
Coordinator
GARCIA VALLVE, SANTIAGO
 E-mail santi.garcia-vallve@urv.cat
franciscojavier.avila@urv.cat
cristina.torres@urv.cat
miriam.ejarque@urv.cat
Lecturers
GARCIA VALLVE, SANTIAGO
ÁVILA ROMÁN, FRANCISCO JAVIER
TORRES FUENTES, CRISTINA
EJARQUE CARBO, MIRIAM
Web http://moodle.urv.net/
General description and relevant information <b>Should there be a health emergency that requires the general public to be confined or which involves restricted mobility during the academic year, we shall attempt to adapt teaching and assessment. Should this situation arise, information about any changes will be given on the Moodle space for every subject.</b><div><b><br /></b><div>This subject studies the general properties of enzymes, their nomenclature and classification, enzymatic purification and the basic principles of enzyme kinetics, the control of enzyme activity and the basics of enzyme catalysis. </div> </div>

Competences
Type A Code Competences Specific
 A3 Have a good knowledge of biochemistry, enzyme kinetics and mechanisms, metabolism and its regulation.
 A6 Know how to design and apply experimental laboratory protocols in the fields of biotechnology, especially chemical, biochemical, microbiological and molecular biology, assessing their risks and safety elements.
Type B Code Competences Transversal
Type C Code Competences Nuclear

Learning outcomes
Type A Code Learning outcomes
 A3 Know the general properties of the enzymes, their nomenclature and classification, the strategies of their purification, and the basic principles of kinetics and enzymatic catalysis.
 A6 Use bioinformatic tools to correlate the structure (proteins and nucleic acids) of a biomolecule with its function.
Type B Code Learning outcomes
Type C Code Learning outcomes

Contents
Topic Sub-topic
1. Introduction Enzyme definition. Historical perspective. Main features of enzymes. Cofactors. Nomenclature and classification of enzymes.
2. Enzymatic kinetics Definition of reaction rate. Measurement of enzymatic activity. Enzymatic kinetics of the reactions of a substrate. Enzymatic inhibition in the reactions of a substrate. Effects of pH and temperature. Enzymatic kinetics of the reactions of two or more substrates.
3. Enzymatic purification Objectives. Purification strategies. Methods of separation. Follow-up purification. Examples of purification.
4. Introduction to Mechanisms of enzymatic action Bases of catalysis. Examples of enzymatic mechanisms. Ribozimes.
5. Regulation of enzyme activity Mechanisms of regulation of enzymatic activity. Cooperativity and allosterism.

Planning
Methodologies  ::  Tests
  Competences (*) Class hours
 Hours outside the classroom
 (**) Total hours
Introductory activities
1 0 1
Lecture
CE3
CE6
 28 42 70
Laboratory practicals
CE3
 12 19 31
Problem solving, exercises
CE3
 14 29 43
Personal attention
1 0 1
 
Mixed tests
A3
A6
 4 0 4
 
(*) On e-learning, hours of virtual attendance of the teacher.
(**) The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies
  Description
Introductory activities The introduction of the subject will take place during the first hour of class where the syllabus, methodology and evaluation of the subject will be presented.
Lecture Presentation of the theoretical contents of each topic.
Laboratory practicals Laboratories. Carrying out experimental processes related to the contents of the subject.
Problem solving, exercises Resolution and evaluation of problems related to the contents of the subject by the students.
Personal attention Attention to any question raised by students.

Personalized attention
Description
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Assessment
Methodologies Competences Description Weight        
Laboratory practicals
CE3
 Attendance is MANDATORY to pass the subject.

Laboratory work and laboratory report. 		15%
Problem solving, exercises
CE3
Problem solving and evaluation. 		15%
Mixed tests
A3
A6
 An exam (40% of the final grade of the subject) and an exam of problems and exercises to solve (30% of the final grade of the subject). 70
Others  
 
Other comments and second exam session

Second call:

It will consist of a unique exam (with a weight of 70%) that will evaluate all the content worked in the subject. The grades from the laboratory practicals and exercises from the first call are kept for the second call.

The exams will be held in person. In case of lockdown or mobility restrictions caused by the Covid-19 health emergency, the assessment activities, including exams, would be done online on the scheduled dates. Updated information can be found on Moodle (virtual teaching space).

During the assessment tests, mobile phones, tablets and other devices not expressly authorized for the test must be switched off and out of sight.

The demonstration of fraudulent conduct of some evaluative activity of a subject in both material: virtual and electronic support, leads to the student the fail of this evaluation activity. Regardless of this, in view of the seriousness of the facts, the center may propose the initiation of a disciplinary file, which will be initiated by resolution of the rector.

Sources of information

Basic Núñez de Castro, I., Enzimología, Última edició disponible, Pirámide
Palmer, T., Understanding enzymes, Última edició disponible, Prentice Hall/Ellis Horwo
Price, N. C.;Stevens, L., Fundamentals of enzymology : the cell and molecular biology of catalytic proteins., Última edició disponible, Oxford University Press.
Nelson, D. K.; Cox, M. M., Lehninger principios de bioquímica , Última edició disponible, Barcelona : Omega, cop.
Donald Voet, Judith G. Voet, Charlotte W. Pratt, Fundamentos de bioquímica : la vida a nivel molecular , Última edició disponible , Médica Panamericana

Complementary , http://www.expasy.org/enzyme/, ,
Mathews, C. K.; Van Holde; K. E., Ahern, K. G. , Bioquímica, Última edició disponible, Addison Wesley
de Arriaga M.D.; Soler J.; et al , Manual de ejercicios de cinética enzimática , Última edició disponible, Universidad de León. Secretariado de Public

Recommendations
(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation.