|
Type A
|
Code |
Competences Specific | | | A3 |
Have a good knowledge of biochemistry, enzyme kinetics and mechanisms, metabolism and its regulation. |
| | A6 |
Know how to design and apply experimental laboratory protocols in the fields of biotechnology, especially chemical, biochemical, microbiological and molecular biology, assessing their risks and safety elements. |
|
Type B
|
Code |
Competences Transversal |
|
Type C
|
Code |
Competences Nuclear |
|
Type A
|
Code |
Learning outcomes |
| | A3 |
Know the general properties of the enzymes, their nomenclature and classification, the strategies of their purification, and the basic principles of kinetics and enzymatic catalysis.
| | | A6 |
Use bioinformatic tools to correlate the structure (proteins and nucleic acids) of a biomolecule with its function.
|
|
Type B
|
Code |
Learning outcomes |
|
Type C
|
Code |
Learning outcomes |
| Topic |
Sub-topic |
| 1. Introduction |
Enzyme definition. Historical perspective. Main features of enzymes. Cofactors. Nomenclature and classification of enzymes. |
| 2. Enzymatic kinetics |
Definition of reaction rate. Measurement of enzymatic activity. Enzymatic kinetics of the reactions of a substrate. Enzymatic inhibition in the reactions of a substrate. Effects of pH and temperature. Enzymatic kinetics of the reactions of two or more substrates. |
| 3. Enzymatic purification |
Objectives. Purification strategies. Methods of separation. Follow-up purification. Examples of purification. |
| 4. Introduction to Mechanisms of enzymatic action |
Bases of catalysis. Examples of enzymatic mechanisms. Ribozimes. |
| 5. Regulation of enzyme activity |
Mechanisms of regulation of enzymatic activity. Cooperativity and allosterism. |
| Methodologies :: Tests |
| |
Competences |
(*) Class hours
|
Hours outside the classroom
|
(**) Total hours |
| Introductory activities |
|
1 |
0 |
1 |
| Lecture |
|
28 |
42 |
70 |
| Laboratory practicals |
|
12 |
19 |
31 |
| Problem solving, exercises |
|
14 |
29 |
43 |
| Personal attention |
|
1 |
0 |
1 |
| |
| Mixed tests |
|
4 |
0 |
4 |
| |
(*) On e-learning, hours of virtual attendance of the teacher.
(**) The information in the planning table is for guidance only and does not take into account the heterogeneity of the students. |
Methodologies
| |
Description |
| Introductory activities |
The introduction of the subject will take place during the first hour of class where the syllabus, methodology and evaluation of the subject will be presented. |
| Lecture |
Presentation of the theoretical contents of each topic. |
| Laboratory practicals |
Laboratories. Carrying out experimental processes related to the contents of the subject. |
| Problem solving, exercises |
Resolution and evaluation of problems related to the contents of the subject by the students. |
| Personal attention |
Attention to any question raised by students. |
|
Description |
|
Time each teacher has reserved to attend and answer questions to students. Due to the health situation for the Covid-19, student care may be carried out through online meetings, at previously arranged times by email, or through other virtual tools. |
|
Methodologies |
Competences
|
Description |
Weight |
|
|
|
|
| Laboratory practicals |
|
Attendance is MANDATORY to pass the subject.
Laboratory work and laboratory report. |
15% |
| Problem solving, exercises |
|
Problem solving and evaluation. |
15% |
| Mixed tests |
|
An exam of objective tests (45% of the final grade of the subject) and an exam of problems and exercises to solve (25% of the final grade of the subject) that will evaluate all the content worked in the subject. It is required a weighted arithmetic mean of the two exams with a minimum grade of 5/10 to pass the subject and to count the grade of the other evaluation activities. |
70% |
| Others |
|
|
|
| |
|
Other comments and second exam session |
|
Second call: It will consist of a unique exam (with a weight of 70%) that will evaluate all the content worked in the subject. It is required a minimum grade of 5/10 in the exam to pass the subject and to count the grade of the other evaluation activities (laboratory practices and problem solving and exercises). The rest of the activities keep the notes and the grade obtained in the first call. During the assessment tests, mobile phones, tablets and other devices not expressly authorized for the test must be switched off and out of sight. The demonstration of fraudulent conduct of some evaluative activity of a subject in both material: virtual and electronic support, leads to the student the fail of this evaluation activity. Regardless of this, in view of the seriousness of the facts, the center may propose the initiation of a disciplinary file, which will be initiated by resolution of the rector. |
| Basic |
Núñez de Castro, I., Enzimología, Última edició disponible, Pirámide
Palmer, T., Understanding enzymes, Última edició disponible, Prentice Hall/Ellis Horwo
Price, N. C.;Stevens, L., Fundamentals of enzymology : the cell and molecular biology of catalytic proteins., Última edició disponible, Oxford University Press.
Nelson, D. K.; Cox, M. M., Lehninger principios de bioquímica , Última edició disponible, Barcelona : Omega, cop.
Donald Voet, Judith G. Voet, Charlotte W. Pratt, Fundamentos de bioquímica : la vida a nivel molecular , Última edició disponible , Médica Panamericana
|
|
| Complementary |
, http://www.expasy.org/enzyme/, ,
Mathews, C. K.; Van Holde; K. E., Ahern, K. G. , Bioquímica, Última edició disponible, Addison Wesley
de Arriaga M.D.; Soler J.; et al , Manual de ejercicios de cinética enzimática , Última edició disponible, Universidad de León. Secretariado de Public
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(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation. |
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