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Type A
|
Code |
Competences Specific | | | A4 |
Know and understand in an integrated manner the organisms at molecular, cellular and metabolic level. |
| | A11 |
Proper application of the diversity of recombinant DNA techniques and methodologies to design cellular genetic engineering strategies for the production of homologous or heterologous proteins or cells capable of acting as biocatalysts |
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Type B
|
Code |
Competences Transversal | | | B4 |
Autonomy, responsibility and initiative |
| | B6 |
Clear and effective communication of information, ideas, problems and solutions in public or a specific technical field |
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Type C
|
Code |
Competences Nuclear |
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Type A
|
Code |
Learning outcomes |
| | A4 |
Use bioinformatic tools to: a) analyze structures and sequences of proteins and nucleic acids, and b) search for information in the main biological and bibliographic databases.
Know advanced tools of genetic engineering, both in prokaryotic and eukaryotic systems.
Appreciate the speed of changes in the knowledge and tools of recombinant DNA technology.
Acquire experience in oral presentation and defense of a task.
| | | A11 |
Use bioinformatic tools to: a) analyze structures and sequences of proteins and nucleic acids, and b) search for information in the main biological and bibliographic databases.
Know the aspects related to the culture of cells in vitro, at a practical, theoretical level and their applications in the field of biotechnology.
Know and master the instrumental analytical techniques that are applied to biotechnology.
Know and master the basic instrumental techniques of biochemistry and molecular biology.
Know advanced tools of genetic engineering, both in prokaryotic and eukaryotic systems.
Appreciate the speed of changes in the knowledge and tools of recombinant DNA technology.
Acquire experience in the oral presentation and defense of a task.
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Type B
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Code |
Learning outcomes |
| | B4 |
Present results in the appropriate way in accordance with the bibliography provided and before the deadline.
Decide how to manage and organize work and time.
| | | B6 |
Plan their communications: generate ideas, search for information, select and order the information, make schemes, determine the type of audience and the objectives of the communication, etc.
Make interesting and persuasive presentations.
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Type C
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Code |
Learning outcomes |
| Topic |
Sub-topic |
| 1. Introduction |
Introduction to genetic engineering to the basic concepts of cloning. Recombinant DNA; vectors and tools; incorporation of DNA to the host, basic techniques (PCR, electrophoresis ...). |
| 2. Vectors for prokaryotes: plasmids and bacteriophages. |
Basic characteristics of plasmid and bacteriophage vectors. Selection. Improvement of the basic vectors. Plasmids pBR322 and pUC. Vectors derived from insertion and replacement lambda phage. Advanced vectors applications: Phage Display. |
| 3. DNA manipulation. |
DNA purification. Cutting and binding: restriction enzymes, ligases, other enzymes used in genetic engineering. |
| 4. Introduction of DNA in the host and selection of recombinants. |
Transformation by chemical and physical methods, calcium chloride method, electroporation. Virus. Identification of transformed organisms. |
| 5. Genomic library |
Construction of genotypes. |
| 6. Yeast cloning |
Cloning in S. cerevisiae. Vectors: chromosomal integration vectors (Yip) and autonomous replication vectors (Yep, YRp, YCp, YAC). Introduction of DNA in yeast. Expression of recombinant proteins. Yeast cell surface display. Applications: Yeast Two Hybrid, Yeast Three Hybrid. |
| 7. Genetic engineering in animal cells. |
Transient and stable transfection. Transfection strategies. Selection methods. Vectors: non-replicating plasmids, plasmids with viral replicons, viral vectors (retroviruses, lentiviruses, adenovirus). Packaging cells. Protein expression. Gene silencing by interference RNA. CRISPR/CAS9 technique. |
| PRACTICAL SYLLABUS |
Cloning of the genome of the Lambda bacteriophage to the vector pUC18 of aplification to E. Coli.
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| Methodologies :: Tests |
| |
Competences |
(*) Class hours
|
Hours outside the classroom
|
(**) Total hours |
| Introductory activities |
|
3 |
0 |
3 |
| Lecture |
|
25 |
40 |
65 |
| Laboratory practicals |
|
30 |
37 |
67 |
| Assignments |
|
2 |
10 |
12 |
| Personal attention |
|
1 |
0 |
1 |
| |
| Mixed tests |
|
2 |
0 |
2 |
| |
(*) On e-learning, hours of virtual attendance of the teacher.
(**) The information in the planning table is for guidance only and does not take into account the heterogeneity of the students. |
Methodologies
| |
Description |
| Introductory activities |
Activities designed to make contact with students, collect information from them and introduce the subject.
|
| Lecture |
Description of the contents of the subject. |
| Laboratory practicals |
Practical application of the theory of a knowledge area in a particular context. Practical exercises in the different laboratories. |
| Assignments |
Seminars: Oral presentation by students of a specific topic or a work (previous written presentation). In-depth work on a topic (monograph). Extension and relationship of the contents given in the master sessions with the professional work.
Laboratory practice report: collection of results and conclusions of laboratory practices. |
| Personal attention |
Reserved time to contact directly with the students outside the class, to solve queries and guide them in the learning process. |
|
Description |
|
Reserved time to contact directly with the students outside the class, to solve queries and guide them in the learning process. |
|
Methodologies |
Competences
|
Description |
Weight |
|
|
|
|
| Laboratory practicals |
|
Individual test of the laboratory practices (20%)
Do a report in groups of the laboratory practices.(15%).
There will be assessed the behavior, interest and compulsory attendance
(5%).
|
40% |
| Assignments |
|
In group seminars. (10%). |
10% |
| Mixed tests |
|
Exam (50%) |
50% |
| Others |
|
|
|
| |
|
Other comments and second exam session |
|
Attendance at laboratory practices is mandatory. You must justify faults. In order to weight all the activities with weight in the final mark, there will be a minimum mark of 5/10 of the practice part and 5/10 of the exam. Second call: you can recover the theory exam and/or the individual test of practices. The group report mark will be saved for the practices and the group seminars. During evaluation tests, mobile phones, tablets and other devices not expressly authorized by the test must be switched off and out of sight. The demonstration of fraudulent conduct of some evaluative activity of some subject in both material and virtual and electronic support leads to the student the suspension note of this evaluation activity. Regardless of this, in view of the seriousness of the facts, the center may propose the initiation of a disciplinary file, which will be initiated by resolution of the rector. |
| Basic |
|
Joseph Sambrook, David W Russell, Molecular Cloning: A Laboratory Manual, 3rd edition, Cold Spring Harbour Laboratory Press, 2001 The most relevant scientific bibliography for the subject will be available in the Moodle space. However, catalogs of suppliers of biological material, enzymes, reagents and devices will be used as a source of specialized and specific information for each seminar, master's session or laboratory experiment. |
| Complementary |
|
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| Subjects that are recommended to be taken simultaneously |
| MOLECULAR GENETICS/19204113 |
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| Subjects that it is recommended to have taken before |
| GENETICS/19204007 | | STRUCTURE AND FUNCTION OF BIOMOLECULES/19204107 | | GENE EXPRESSION AND REPLICATION/19204102 |
|
(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation. |
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