IDENTIFYING DATA 2017_18
Subject (*) MICROBIAL MOLECULAR BIOTECHNOLOGY Code 19204121
Study programme
Bachelor's Degree in Biotechnology (2009)
 Cycle 1st
Descriptors Credits Type Year Period
4 Compulsory Third 2Q
Language
Català
Department Biochemistry and Biotechnology
Coordinator
CORDERO OTERO, RICARDO ROMÁN
 E-mail ricardo.cordero@urv.cat
Lecturers
CORDERO OTERO, RICARDO ROMÁN
Web
General description and relevant information The objectives of this subject is to give students a critical and creative vision of the use of microorganisms developed by genetic engineering in applications of biotechnology relevance, such as food production, industrial application enzymes, fuels, health human, etc.

Competences
Type A Code Competences Specific
 A8 Analyse appropriately data and experimental results from the fields of biotechnology with statistical techniques and be able to interpret it.
 A9 Know in depth the microorganisms, both prokaryotes and eukaryotes, viruses, as well as the diversity of metabolisms present in prokaryotes, and their possibilities of biotechnological use.
 A15 Know the diversity of existing biotechnological processes and products, as well as the recent biotechnological advances, and know how to explain them and communicate them to diverse audiences.
Type B Code Competences Transversal
 B3 Critical, logical and creative thinking, and an ability to innovate
Type C Code Competences Nuclear
 C3 Be able to manage information and knowledge

Learning outcomes
Type A Code Learning outcomes
 A8 Know the molecular biology of systems to its genomic, transcriptomic, proteomic and metabolomic aspects.
 A9 Metabolically modify biotechnological microbian models in the food, medicine and agriculture sectors.
 A15 Master the techniques of molecular biology used to obtain genetically modified animals and plants, and know the possibilities of applying molecular biotechnology to animals and plants.
Type B Code Learning outcomes
 B3 Put forward new ideas, opportunities or solutions to familiar problems and/or processes.
Type C Code Learning outcomes
 C3 Locate and access information effectively and efficiently.

Contents
Topic Sub-topic
1. Microbial biotechnology: theoretical concepts, history and areas of incidence
2. Availability and selection of microbial biotechnology models
3. Genetic engineering of microbial metabolism
4. Techniques for the creation and selection of microbial mutants with biotechnological interest
5. Biotechnological strategies in the development of bacteria for industrial and agricultural use
6. Microorganisms as producers of biomolecules
7. Biotechnologically developed microorganisms for the aromas and biofuels industry
8. Yeast as a model and tool in the knowledge of the cell higher eukaryotes and their application in medicine

Planning
Methodologies  ::  Tests
  Competences (*) Class hours
 Hours outside the classroom
 (**) Total hours
Introductory activities
2 0 2
Lecture
A9
A15
 25 37 62
Problem solving, classroom exercises
B3
 4 0 4
Laboratory practicals
A8
B3
C3
 20 10 30
Personal tuition
2 0 2
 
 
(*) On e-learning, hours of virtual attendance of the teacher.
(**) The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies
  Description
Introductory activities In the theoretical classes will be exposed the scientific and practical foundations for the comprehension of the bibliography that the students must present in the second part of the following class. In this way, only the first theoretical class will not count on the active participation of the students.
Lecture From the second class the students will be proposed the following dynamic: 40 min will be occupied by the teacher to teach the basic concepts of the bibliography to be presented by the students. In the second part of the next class: 25 min for the presentation of an article by the students, 15 min for the questions by the audience, 25 min for the presentation of a second article by the students and 15 min for questions by the audience.
Problem solving, classroom exercises The responsible students for the exhibition must present on the website of the subject the possible answers using any of the existing supports on the internet.
Laboratory practicals Design to develop genetic modifications of microorganisms and evaluate them at the molecular and physiological level.
Personal tuition The students will have a weekly consultation schedule to solve specific doubts of the subject.

Personalized attention
Description
The students will have a weekly consultation schedule to solve specific doubts of the subject.

Assessment
Methodologies Competences Description Weight        
Lecture
A9
A15
 Individual written questionnaire of a scientific article similar to those made in class 30%
Problem solving, classroom exercises
B3
 The ability to discuss and answer questions about the topics taught during the lectures. 20%
Laboratory practicals
A8
B3
C3
 Recognition of the critical points for the development of genetically modified microorganisms. 50%
Others  
 
Other comments and second exam session

The second call will consist of an examination of all the contents of the subject.

The demonstration of the fraudulent conduct of some evaluative activity of a subject in both material and virtual or electronic support leads to the student the failing grade of this evaluation activity. Regardless of this, in view of the seriousness of the facts, the centre may propose the initiation of a disciplinary file, which will be initiated by resolution of the rector.

Sources of information

Basic , Recombinant Gene Expression, Methods in Molecular Biology Volume 824, 2012
, Recombinant Protein Production in Yeast: Methods and Protocols, Series: Methods in Molecular Biology, Volume: 866, 2012
Satyanarayana, T., Yeast Biotechnology: Diversity and Applications, Kunze, Gotthard (Eds.), 2009
Alexander N. Glazer and Hiroshi Nikaido, Microbial Biotechnology “Fundamentals of Applied Microbiology”, University of California, Berkeley, 2007
Lee Yuan Kun, Microbial Biotechnology “Principles and Applications”, World Scientific Publishing Co. Pte Ltd, 2006
Jose-luis Barredo, Methods in Biotechnology “Microbial Processes and Products”, Humana Press Inc, 2005
Walker, Graeme M, Yeast - Physiology and Biotechnology, John Wiley & Sons, 1998

Complementary

Recommendations

Subjects that continue the syllabus
ANIMAL AND PLANT BIOTECHNOLOGY/19204122
FROM MODEL ORGANISMS TO HUMAN BIOLOGY/19204218


Subjects that it is recommended to have taken before
BIOCHEMISTRY/19204008
CELL BIOLOGY/19204006
METABOLISM OF MICROORGANISMS/19204110
(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation.