IDENTIFYING DATA 2018_19
Subject (*) PROTEIN ENGINEERING Code 19204222
Study programme
Bachelor's Degree in Biotechnology (2009)
 Cycle 1st
Descriptors Credits Type Year Period
3 Optional 1Q
Language
Català
Department Biochemistry and Biotechnology
Coordinator
PUJADAS ANGUIANO, GERARD
 E-mail gerard.pujadas@urv.cat
Lecturers
PUJADAS ANGUIANO, GERARD
Web http://moodle.urv.cat
General description and relevant information he subject gives the bases (theoretical and practical) to understand what are the properties of a protein susceptible to be improved, how to make these improvements and predict the effect of the changes made in the protein sequence on its structure.

Competences
Type A Code Competences Specific
 A8 Analyse appropriately data and experimental results from the fields of biotechnology with statistical techniques and be able to interpret it.
Type B Code Competences Transversal
 B5 Teamwork, collaboration and sharing of responsibility
 B6 Clear and effective communication of information, ideas, problems and solutions in public or a specific technical field
Type C Code Competences Nuclear

Learning outcomes
Type A Code Learning outcomes
 A8 Use different databases (e.g., PDB, SCOP, ...) to find out what are the main characteristics of the structure of a given protein.
Use computer programs to analyze the structure of proteins.
Type B Code Learning outcomes
 B5 Ensure that the way they communicate and relate to others contributes to the cohesion of the group.
Take an interest in the importance of the group’s activity.
 B6 Understand that non-verbal language is appropriate to verbal discourse.
Plan their communications: generate ideas, search for information, select and order the information, make schemes, determine the type of audience and the objectives of the communication, etc.
Type C Code Learning outcomes

Contents
Topic Sub-topic
1. Three-dimensional structure of proteins 1.1. The SPdbV/SwissModel binomial as computational tools in the field of protein engineering.

1.2. Proteins with known structure: analysis of their 3D structure.
2. Fundamentals of protein engineering 2.1. Application of protein engineering to introduce mutations that improve the thermostability of an enzyme.

2.2. Application of protein engineering in order to introduce mutations that adapt the activity of an enzyme at low temperatures.
3. De novo protein design

Planning
Methodologies  ::  Tests
  Competences (*) Class hours
 Hours outside the classroom
 (**) Total hours
Introductory activities
1 0 1
Lecture
A8
 10 15 25
IT-based practicals in computer rooms
A8
 20 25 45
Personal attention
2 0 2
 
Short-answer objective tests
A8
 2 0 2
 
(*) On e-learning, hours of virtual attendance of the teacher.
(**) The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies
  Description
Introductory activities Presentation of the subject (with details of the teaching methodology to be used and the assessment criteria)
Lecture Theoretical explanations that will be carried out in the computer room and which will be followed by a practical (non-evaluable) exercise by the students or the visualization of a video in order to exemplify the theoretical explanations
IT-based practicals in computer rooms Practical exercise which consists (in groups of three or four students), to do a practical exercise of analysis by computational methods of the structure of a protein, and then ask the students, based on this analysis, to suggest what changes could be made in their sequence for improve some property (eg, thermostability, etc.) with respect to the native protein.
Personal attention Students can consult their questions directly to the teacher going to their office (at 106) during the visit hours (see institutional website) or also sending an email from moodle.

Personalized attention
Description
Students can consult their questions directly to the teacher going to their office (at 106) during the visit hours (see institutional website) or also sending an email from moodle.

Assessment
Methodologies Competences Description Weight        
IT-based practicals in computer rooms
A8
 Practical exercise where, in groups of three or four students, a practical exercise of analysis by computational methods of the structure of a protein will be carried out and they will be asked that, based on this analysis, they suggest what changes are they could perform in their sequence in order to improve some property (eg, thermostability, etc.) with respect to the native protein Aquestes pràctiques poden proporcionar un màxim de 5/10 en la qualificació final de l'assignatura.

Estas prácticas pueden proporcionar un máximo de 5/10 en la calificación final de la asignatura.

These practices can contribute a maximum of 5 points out of 10 to the final grade of the subject.
Short-answer objective tests
A8
 A single test where the student will have to respond to a series of short objective questions that demonstrate the level of individual benefit that has been achieved in the subject Aquesta prova objectiva pot proporcionar un màxim de 5/10 en la qualificació final de l'assignatura.

Esta prueba objetiva puede proporcionar un máximo de 5/10 en la calificación final de la asignatura.

This test can provide a maximum of 5 points out of 10 to the final grade of the subject.
Others  
 
Other comments and second exam session

In the second call only the equivalent part of the objective tests of short questions of the first call (with identical weight in the final note) will be carried out. This means that the note obtained in the "Practicals using information and communication technologies (ICTs) in computer rooms" section of the first call will be valid also for the second call.

During the evaluation tests, mobile phones, tablets and other devices that are not expressly authorized for the test, must be turned off and out of sight. The demonstrable fraudulent realization of some evaluation activity of a subject both in material and virtual and electronic support entails the student's suspense note of this evaluation activity. Regardless of this, given the seriousness of the events, the center may propose the initiation of a disciplinary file, which will be opened by resolution of the rector.

Sources of information

Basic Lesk, Arthur M., Introduction to protein science: architecture, function, and genomics, 2nd ed., 2010
Edited by Sheldon J. Park, Jennifer R. Cochran, Protein engineering and design, , 2010
Carlos Gómez-Moreno Calera y Javier Sancho Sanz, Estructura de proteínas, , 2003

Complementary

Recommendations


Subjects that it is recommended to have taken before
STRUCTURE AND FUNCTION OF BIOMOLECULES/19204107
(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation.