IDENTIFYING DATA 2012_13
Subject (*) VINEGAR MAKING: TECHNOLOGICAL AND MICROBIOLOGICAL ASPECTS Code 19605208
Study programme
Oenology (2010)
Cycle 2nd
Descriptors Credits Type Year Period
3 Optional Only annual
Language
Anglès
Department Bioquímica i Biotecnologia
Enginyeria Química
Coordinator
MAS BARON, ALBERTO
TORIJA MARTÍNEZ, MARÍA JESÚS
E-mail francisco.lopez@urv.cat
mjesus.torija@urv.cat
0
Lecturers
LÓPEZ BONILLO, FRANCISCO
TORIJA MARTÍNEZ, MARÍA JESÚS
MATEO ALESANCO, ESTÍBALIZ
Web
General description and relevant information Study of the vinegar making process both from a microbiological and technological point of view

Competences
Type A Code Competences Specific
  Common
  AC2 Scientific and technical knowledge of the wine making processes.
  Professional
  Research
  AR5 The ability for exhaustive bibliographic searches in highly specialized oenological topics.
  AR7 The presentation of results in a scientific way, according to the accepted standards.
  AR8 The critical evaluation of results of oenological research.
Type B Code Competences Transversal
  Common
  BC2 Work in an autonomous way with initiative.
  BC3 Flexibility. Availability to adapt to changing environments.
  BC4 Capacity to solve problems in an effective way.
  BC6 Ability to act with a critical and responsible spirit.
Type C Code Competences Nuclear
  Common
  CC2 Use of specific tools of Information and Knowledge Technologies for the professional development derived from the postgraduate course.

Learning aims
Objectives Competences
Knowledge of the different methods for the production of vinegar and the possible actions to improve/optimize the acetification process AC2
BC3
BC4
CC2
Learning the techniques needed to microbiologically control the acetification process AR5
AR7
AR8
BC4
BC6
CC2
Finding out the molecular techniques for the identification of the microorganisms responsible for the process AR7
AR8
BC2
BC4
CC2

Contents
Topic Sub-topic
Topic 1: Introduction * Definition of wine vinegar
* Regulation
* Evolution and economic importance
Topic 2: Acetic acid bacteria * General
* Factors affecting the growth
* Metabolism
* Taxonomy
* Identification methods:
Isolation and enumeration
Molecular techniques
Topic 3: Methods for vinegar production * Traditional Methods
* Industrial Methods
* Vinegar Treatments
Topic 4: Monitoring and control of acetification process * Evolution of acetic and alcoholic degree
* Ecological studies. Microbiota involved
Topic 5: Traditional aging in wood No subtopics
Topic 6: Sensory and chemical analysis * Vinegar quality parameters
* Chemical analysis of vinegar
* Sensory analysis of vinegar

Planning
Methodologies  ::  Tests
  Competences (*) Class hours Hours outside the classroom (**) Total hours
Introductory activities
1 0 1
 
Lecture
15 3 18
Presentations / expositions
3 15 18
Assignments
0 25 25
Debates
2 10 12
 
Personal tuition
1 0 1
 
 
(*) On e-learning, hours of virtual attendance of the teacher.
(**) The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies
  Description
Introductory activities Introduction to the subject and to the evaluation criteria
Lecture Explanation of course contents
Presentations / expositions Oral presentation about a scientific paper related to the subject
Assignments Students will choose a topic related to enology or vinegar elaboration and will perform a bibliographical review about this topic
Debates Students will discuss about technical or microbiological aspects of vinegar elaboration
Personal tuition

Personalized attention
 
Personal tuition
Description
The students may contact teachers by email, or personally with an appointment

Assessment
  Description Weight
Lecture Answering questions to show the degree of understanding of the course content 25
Presentations / expositions Oral presentation of a paper related to the production of vinegar 25
Assignments Common work to different subjects of Enological biotechnology, giving special relevance to the issues related to the production of vinegar 25
Debates Response to technical problems of vinegar elaboration 25
 
Other comments and second exam session

Sources of information

Basic M.R. ADAMS, Vinegar, 1998, Blake Academic and Professional
AENOR, Análisis Sensorial. Tomo I, 1997, AENOR
C. LLAGUNO MARCHENA y M.C. POLO, El vinagre de vino, 1991, CSIC
H. EBNER y H. FOLLMANN, Acetic acid. Chapter 3b on Biotechnology, 1983, H.J. Rehm y G. Reed
F. MECCA, R. ANDREOTTI, L. VERONELLI, L'aceto, 1979, AEB

Complementary Achaerandio, I.; Güell, C.; Jiménez, L.; Mateo, J.M.; López, F., Caracterización de vinagres comerciales mediante análisis multivariable, 2002, Tecnología del Vino, Num.3, 103-107
Calviño, J., Achaerandio, I., Labbé M., García-Llobonadín, L., Güell, C., López,, Application of denaturing gradient gel electrophoresis (DGGE) analysis to evaluate acetic acid bacteria in traditional balsamic vinegar, 2006, . Food Microbio. 23, 809-813.
De Vuyst, L., Camu, N. , De Winter, T., Vandemeulebroecke, K. , Van de Perre, V. , Vancanneyt, M.,, Validation of the (GTG)5-rep-PCR fingerprinting technique for rapid classification and identification of acetic acid bacteria, with a focus on isolates from Ghanaian fermented cocoa beans, 2007, Int J Food Microbiol. doi:10.1016/j.ijfoodmicro.2
Du Toit, W.J. & Pretorius, I.J., The occurrence, control and esoteric effect of acetic acid bacteria in winemaking, 2002, Ann Microbiol 52, 155-179.
González, A., Guillamón, J.M., Mas A., Poblet, M., Application of molecular methods for routine identification of acetic acid bacteria, 2006a, Int J Food Microbiol 108, 141-146.
González, A., Hierro, N., Poblet, M., Mas, A. & Guillamón, J.M., Enumeration and detection of acetic acid bacteria by real-time and nested polymerase chain reactions, 2006b, FEMS Microbiol Lett 254, 123-128
Gullo, M., Caggia, C., De Vero, L. & Giudici, P., Characterisation of acetic acid bacteria in “traditional balsamic Vinegar”, 2006, Int J Food Microbiol 106, 209-212
Poblet, M., Rozès, N., Guillamón, J.M. & Mas, A., Identification of acetic acid bacteria by restriction fragment length polymorphism analysis of a PCR-amplified fragment of the gene coding for 16S rRNA, 2000, Lett Appl Microbiol 31, 63-67
Achaerandio, I.; Güell, C.; López, F., New approach to continuous vinegar decolourisation with exchange resins, 2007, Journal of Food Engineering, 78(3), 991-994
López, F.; Pescador, P.; Güell, C.; Morales, M.L.; García-Parrilla, M.C.; Troncoso, A.M., Industrial vinegar clarification by cross-flow microfiltration: effect on colour and polyphenol content, 2005, Journal of Food Engineering, 68(1), 133-136
Ruiz, A., Poblet, M., Mas, A. & Guillamon, JM., Identification of acetic acid bacteria by RFLP of PCR-amplified 16S rDNA and 16S-23S rDNA intergenic spacer, 2000, Int J Syst Evol Microbiol 50, 1981-1987
Tesfaye, W., García-Parrilla, M.C., & Troncoso, A.M., Sensory evaluation of Sherry wine vinegar, 2002, Journal of Sensory Studies, 17 (2), 133-144
Yamada Y., Yukphan P., Review. Genera and species in acetic acid bacteria, 2008, Int J Food Microbiol. doi:10.1016/j.ijfoodmicro.20

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(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation.