IDENTIFYING DATA 2012_13
Subject (*) MOLECULAR TECHNIQUES FOR MICROBIOLOGICAL ANALYSIS AND CONTROL Code 19605213
Study programme
Oenology (2010)
 Cycle 2nd
Descriptors Credits Type Year Period
3 Optional Only annual
Language
Anglès
Department Bioquímica i Biotecnologia
Coordinator
REGUANT MIRANDA, CRISTINA
 E-mail mariafrancesca.fort@urv.cat
cristina.reguant@urv.cat
mjesus.torija@urv.cat
Lecturers
FORT MARSAL, MARIA FRANCESCA
REGUANT MIRANDA, CRISTINA
TORIJA MARTÍNEZ, MARÍA JESÚS
Web
General description and relevant information Study of techniques based on molecular methods of analysis and microbiological control applied to oenology.

Competences
Type A Code Competences Specific
  Common
  AC2 Scientific and technical knowledge of the wine making processes.
  Professional
  Research
  AR2 Appropriate training to incorporate to a university department in order to pursue a PhD.
  AR4 Excellence in the study and knowledge in the oenology research.
  AR7 The presentation of results in a scientific way, according to the accepted standards.
  AR8 The critical evaluation of results of oenological research.
Type B Code Competences Transversal
  Common
  BC2 Work in an autonomous way with initiative.
  BC6 Ability to act with a critical and responsible spirit.
  BC12 Learning to learn.
Type C Code Competences Nuclear
  Common
  CC2 Use of specific tools of Information and Knowledge Technologies for the professional development derived from the postgraduate course.
  CC4 Development of informational skills.

Learning aims
Objectives Competences
Knowledge of the molecular biology techniques used in the identification of wine microorganisms AR2
AR4
AC2
 BC2
BC12
 CC2
Ability to analyse and discuss the results obtained by the identification techniques studied AR7
AR8
 BC2
BC6
 CC2
CC4

Contents
Topic Sub-topic
1. Introduction to the application of molecular techniques for the control of wine microorganisms 1.1. Classical identification methods versus molecular methods
1.2. Culture-dependent and -independent methods: advantages and disadvantages
2. Culture-dependent methods for the identification and characterization of wine microorganisms 2.1. Sequencing and analysis of genes
2.2. Identification based on restriction profiles
2.3. Characterization by PCR-based polymorphisms: RAPD, ERIC,
REP - PCR
3. Culture-independent methods for the identification of wine microorganisms 3.1. Real Time PCR
3.2. DGGE / TGGE
3.3. FISH

Planning
Methodologies  ::  Tests
  Competences (*) Class hours Hours outside the classroom (**) Total hours
Introductory activities
1 0 1
 
Lecture
20 20 40
Assignments
0 14 14
Presentations / expositions
19 0 19
 
Personal tuition
1 0 1
 
 
(*) On e-learning, hours of virtual attendance of the teacher.
(**) The information in the planning table is for guidance only and does not take into account the heterogeneity of the students.

Methodologies
Methodologies
  Description
Introductory activities Overview of contents.
Description of evaluation criteria.
Lecture Explanation of course contents
Assignments Works related to the course contents to be developed by the students.
Presentations / expositions Oral presentation of the work done in the classroom.
Personal tuition

Personalized attention
 
Presentations / expositions
Assignments
Personal tuition
Description
Advice on the tasks to do throughout the course.

Assessment
  Description Weight
Assignments Answering of questions about Molecular techniques reviews

Answering of questions to review the contents exposed in the lectures 		20%



25%
Presentations / expositions Presentation of a scientific paper related to course content.

Presentation of a bibliographical review 		30%



25%
 
Other comments and second exam session

Sources of information

Basic

·     Beltran, G , Torija, M.J., Novo, M., Ferrer, N., Poblet, M., Guillamon, J.M., Rozès, N. y Mas, A. (2002) Analysis of yeast populations during alcoholic fermentation: a six year follow-up study. Systematic and Applied Microbiology 25: 287-293.

·     Blasco, L., Ferrer, S. y Pardo, I.(2003) Development of specific fluorescent oligonucleotide probes for in situ identification of wine lactic acid bacteria. FEMS Microbiol. Lett., 225, 115-123.

·     Daniel, H.M. y Meyer, W. (2003) Evaluation of ribosomal RNA and actin gene sequences for the identification of ascomycetous yeasts. International Journal of Food Microbiolology 86, 61-78.

·     Esteve-Zarzoso, B., Belloch, C., Uruburu, F. y Querol, A., (1999). Identification of yeasts by RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers. International Journal of Systematic  Bacteriolology 49, 329-337.

·     González, Á. , N. Hierro, M. Poblet, N. Rozès, A. Mas y J. M. Guillamón. (2004).

Application of molecular methods for the differentiation of acetic acid bacteria in a red wine fermentation. Journal of Applied Microbiology 96: 853-860

·     González, A., Guillamón, J.M., Mas A. y Poblet, M., (2006). Application of molecular methods for routine identification of acetic acid bacteria. Int J Food Microbiol 108, 141-146.

·     Querol, A., E. Barrio, T. Huerta, y D. Ramón. 1992. Molecular monitoring of wine fermentations conducted by active dry yeast strains. Applied and Environmental Microbiology  58:2948-2953.

·     Reguant, C , A Bordons. (2003) Typification of Oenococcus oeni strains by multiplex RAPD-PCR and study of population dynamics during malolacic fermentation. Journal of Applied Microbiology, 95, 2: 344-353.

·     Rodas, A.M., Ferrer, S. y Pardo, I.(2003) 16S-ARDRA, a tool for identification of lactic acid bacteria isolated from grape must and wine. Syst. Appl. Microbiol., 26, 412-422.

·     Ruiz, M. Poblet, A. Mas y J.M. Guillamón. (2000) Identification of acetic acid bacteria by RFLP of the PCR-amplified 16S rDNA and 16S-23S rDNA intergenic spacers. International Journal of Systematic and Evolutionary Microbiology, 50: 1981-1987

·     Sabaté, J, J Cano, B Esteve-Zarzoso y JM Guillamón. (2002) Isolation and identification of yeasts associated with vineyard and winery by RFLP analysis of ribosomal genes and mitochnrial DNA. Microbiological Research 157: 1-8.

·     Torija. M.J., Rozès, N., Poblet, M., Guillamón, J.M. y Mas, A. (2001) Yeast population dynamics in spontaneous fermentations: Comparison between two different wine-producing areas over a period of three years. Antonie van Leeuwenhoek 79:345-352.

·     Zapparoli, G, C Reguant, A Bordons, S Torriani, F Dellaglio. (2000) Genomic DNA fingerprinting of Oenococcus oeni strains by pulsed-field gel electrophoresis and randomly PCR-amplified polymorphic DNA. Current  Microbiology 40: 351-355.

·     Zapparoli, G., Torriani, S., Pesente, P., Dellaglio, F. (1998) Design and evaluation of malolactic enzyme gene targeted primers for rapid identification and detection of Oenococcus oeni in wine. Letters in Applied Microbiology, 27, 243-246.
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(*)The teaching guide is the document in which the URV publishes the information about all its courses. It is a public document and cannot be modified. Only in exceptional cases can it be revised by the competent agent or duly revised so that it is in line with current legislation.