Guia docent Escola Tècnica Superior d'Enginyeria |
català |
Doble titulació de Grau d'Enginyeria Informàtica i de Biotecnologia (2014) |
Assignatures |
BIOTECNOLOGIA MOLECULAR ANIMAL I VEGETAL |
Continguts |
DADES IDENTIFICATIVES | 2016_17 |
Assignatura | BIOTECNOLOGIA MOLECULAR ANIMAL I VEGETAL | Codi | 17914125 | |||||
Ensenyament |
|
Cicle | 1r | |||||
Descriptors | Crèd. | Tipus | Curs | Període | ||||
6 | Obligatòria | Tercer | 2Q |
Competències | Resultats d'aprenentage | Continguts |
Planificació | Metodologies | Atenció personalitzada |
Avaluació | Fonts d'informació | Recomanacions |
Tema | Subtema |
VEGETAL TEMA 1. Introducció a la biotecnologia vegetal | Definicions Dades de producció Evolució de la millora vegetal Aplicacions |
VEGETAL TEMA 2. Desenvolupament dels vegetals | Teixits de les plantes Definicions Control del cicle cel·lular vegetal Embriogènesi Meristems |
VEGETAL TEMA 3.Cultius cel·lulars vegetals | Cultius in vitro vegetals Etapes Aplicacions Explants |
VEGETAL TEMA 4.Variació somaclonal | Definició Factors Avantatges i inconvenients Mètodes de detecció |
VEGETAL TEMA 5. Agrobacterium tumefaciens | Definició Plàsmid Ti Mecanisme d'actuació Aplicació en engenyeria genètica |
VEGETAL TEMA 6. Tècniques de transferència gènica en vegetals | Limitacionss d' Agrobacterium Tècnicas de transferència directa Transformació de protoplasts Biolística Electroporació Microinyecció Whiskers |
Animal Topic 1 Introduction to animal biotechnology |
Definition and objectives Historical facts The technologies involved Applications Regulation and concerns |
Animal Topic 2 Animals as laboratory tools |
Natural and laboratory populations Key principles of 3Rs Mouse as model system Genetic standardization Inbred strains, F1 hybrids, coisogenic, congenic and outbred strains among others Mouse nomenclature Genetic drift and substrain development Genetic integrity monitoring and SNPs Cryopreservation |
Animal Topic 3 Early embryogenesis and reproductive biology in laboratory mouse |
Preformism versus epigenesis Stages of embryogenesis Spermatogenesis Oogenesis Fertilization Acrosomal and cortical reaction Ca flux Zona pellucida Segmentation and gastrulation Morula and blastula Ectoderm, endoderm and mesoderm Organogenesis Reproductive characteristics Sex determination Male anatomy and sperm production Female anatomy and egg production Estrous cycle Mating options Problems |
Animal Topic 4 Assisted reproduction technologies (ARTs) in human and animals |
Assisted reproduction technologies (ARTs) Human infertility Causes of female and male infertility Controled ovarian hyperstimulation (COH) and artificial insemination (AI) Cryopreservation- egg, sperm and embryo banking Sex determination In vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) Preimplantation genetic diagnostics (PGD) |
Animal Topic 5 Cloning |
Reproductive cloning Embryos and biotechnology Reproductive and therapeutic cloning History of cloning Embryo splitting Somatic cell nuclear transfer (SCNT) Dolly Problems and difficulties Cloning of endangered and extinct species Therapeutic cloning Regenerative medicine and therapeutic cloning Stem cells Adult stem cells Embryonic stem (ES) cells Somatic cell nuclear transfer (SCNT) stem cells Induced pluripotent stem cells (iPS cells) Stem cell-based therapies |
Animal Topic 6 Non-targeted transgenesis |
Physiological, pathological and induced models Forward versus reverse genetics Non-targeted transgenesis Pronuclear injection (PNI) Viral transgenesis Sperm-mediated transgenesis |
Animal Topic 7 Targeted transgenesis |
Gene targeting Mouse embryonic stem (ES) cells Homologous recombination Positive and negative selection of ES cell clones Typical gene targeting experiment Blastocyst injection Chimera production and breeding YAC transgenes Knock out (KO) and Knock in (KI) Conditional strategies Cre/loxP recombination system Flp/Frt system Inducible strategies Cre-ERT system TetOn/TetOff systems Reporter mouse RNAi |
Animal Topic 8 Applications in transgenic livestock |
Transgenic livestock Methodologies Pharming (biopharming) Applications in agriculture: enhanced nutrition, reduced environmental impact, enhanced milk, enhanced growth rates and carcass composition, improved disease resistance, improved reproductive performance and fecundity, improved hair and fiber Risks and conclusions |
Animal Topic 9 Transgenesis and pathology |
Non-targeted transgenesis and HER2 TG mice in breast cancer Targeted transgenesis, colon stem cells and colon cancer Aging Hutchinson-Gilford progeria syndrome (HGPS) |